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basic fibroblast growth factor  (R&D Systems)


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    R&D Systems basic fibroblast growth factor
    mRNA expression and protein expression of PTEN in normal astrocyte or GBM cell lines, and inhibition by rNDV-PTEN of cell viability and migration by inducing apoptotic cell death in U87-MG cells. PTEN (A) mRNA and (B) protein expression in normal astrocyte (CCF-STTG1) and GBM cell lines (U87-MG, T98G, Proneural <t>X01</t> and Mesenchymal 83). CCF-STTG1 cells and U87-MG cells were infected rNDV or rNDV-PTEN 1 MOI for 36 h. (C) Cell viability assay performed using CCF-STTG1 cells and GBM cell lines with rNDV or rNDV-PTEN 1 MOI treatment using a Cell Counting Kit-8 Kit. (D) U87-MG cells were infected with rNDV or rNDV-PTEN and a Transwell assay was performed to assess cell migration. Cells migrated from the upper chamber to the lower chamber were stained with DAPI. Scale bar, 50 µm. (E) Apoptosis (DNA fragmentation) in U87-MG cells measured using TUNEL staining after virus infection. *P<0.05 vs. CCF-STTG1 or CON. PTEN, phosphatase and tensin homolog; GBM, glioblastoma; rNDV, recombinant Newcastle disease virus; MOI, multiplicity of infection; CON, control; n.s., not significant.
    Basic Fibroblast Growth Factor, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/basic fibroblast growth factor/product/R&D Systems
    Average 95 stars, based on 101 article reviews
    basic fibroblast growth factor - by Bioz Stars, 2026-03
    95/100 stars

    Images

    1) Product Images from "Anticancer effect of the oncolytic Newcastle disease virus harboring the PTEN gene on glioblastoma"

    Article Title: Anticancer effect of the oncolytic Newcastle disease virus harboring the PTEN gene on glioblastoma

    Journal: Oncology Letters

    doi: 10.3892/ol.2024.14752

    mRNA expression and protein expression of PTEN in normal astrocyte or GBM cell lines, and inhibition by rNDV-PTEN of cell viability and migration by inducing apoptotic cell death in U87-MG cells. PTEN (A) mRNA and (B) protein expression in normal astrocyte (CCF-STTG1) and GBM cell lines (U87-MG, T98G, Proneural X01 and Mesenchymal 83). CCF-STTG1 cells and U87-MG cells were infected rNDV or rNDV-PTEN 1 MOI for 36 h. (C) Cell viability assay performed using CCF-STTG1 cells and GBM cell lines with rNDV or rNDV-PTEN 1 MOI treatment using a Cell Counting Kit-8 Kit. (D) U87-MG cells were infected with rNDV or rNDV-PTEN and a Transwell assay was performed to assess cell migration. Cells migrated from the upper chamber to the lower chamber were stained with DAPI. Scale bar, 50 µm. (E) Apoptosis (DNA fragmentation) in U87-MG cells measured using TUNEL staining after virus infection. *P<0.05 vs. CCF-STTG1 or CON. PTEN, phosphatase and tensin homolog; GBM, glioblastoma; rNDV, recombinant Newcastle disease virus; MOI, multiplicity of infection; CON, control; n.s., not significant.
    Figure Legend Snippet: mRNA expression and protein expression of PTEN in normal astrocyte or GBM cell lines, and inhibition by rNDV-PTEN of cell viability and migration by inducing apoptotic cell death in U87-MG cells. PTEN (A) mRNA and (B) protein expression in normal astrocyte (CCF-STTG1) and GBM cell lines (U87-MG, T98G, Proneural X01 and Mesenchymal 83). CCF-STTG1 cells and U87-MG cells were infected rNDV or rNDV-PTEN 1 MOI for 36 h. (C) Cell viability assay performed using CCF-STTG1 cells and GBM cell lines with rNDV or rNDV-PTEN 1 MOI treatment using a Cell Counting Kit-8 Kit. (D) U87-MG cells were infected with rNDV or rNDV-PTEN and a Transwell assay was performed to assess cell migration. Cells migrated from the upper chamber to the lower chamber were stained with DAPI. Scale bar, 50 µm. (E) Apoptosis (DNA fragmentation) in U87-MG cells measured using TUNEL staining after virus infection. *P<0.05 vs. CCF-STTG1 or CON. PTEN, phosphatase and tensin homolog; GBM, glioblastoma; rNDV, recombinant Newcastle disease virus; MOI, multiplicity of infection; CON, control; n.s., not significant.

    Techniques Used: Expressing, Inhibition, Migration, Infection, Viability Assay, Cell Counting, Transwell Assay, Staining, TUNEL Assay, Virus, Recombinant, Control



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    basic fibroblast growth factor  (R&D Systems)
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    R&D Systems basic fibroblast growth factor
    mRNA expression and protein expression of PTEN in normal astrocyte or GBM cell lines, and inhibition by rNDV-PTEN of cell viability and migration by inducing apoptotic cell death in U87-MG cells. PTEN (A) mRNA and (B) protein expression in normal astrocyte (CCF-STTG1) and GBM cell lines (U87-MG, T98G, Proneural <t>X01</t> and Mesenchymal 83). CCF-STTG1 cells and U87-MG cells were infected rNDV or rNDV-PTEN 1 MOI for 36 h. (C) Cell viability assay performed using CCF-STTG1 cells and GBM cell lines with rNDV or rNDV-PTEN 1 MOI treatment using a Cell Counting Kit-8 Kit. (D) U87-MG cells were infected with rNDV or rNDV-PTEN and a Transwell assay was performed to assess cell migration. Cells migrated from the upper chamber to the lower chamber were stained with DAPI. Scale bar, 50 µm. (E) Apoptosis (DNA fragmentation) in U87-MG cells measured using TUNEL staining after virus infection. *P<0.05 vs. CCF-STTG1 or CON. PTEN, phosphatase and tensin homolog; GBM, glioblastoma; rNDV, recombinant Newcastle disease virus; MOI, multiplicity of infection; CON, control; n.s., not significant.
    Basic Fibroblast Growth Factor, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/basic fibroblast growth factor/product/R&D Systems
    Average 95 stars, based on 1 article reviews
    basic fibroblast growth factor - by Bioz Stars, 2026-03
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    Image Search Results


    mRNA expression and protein expression of PTEN in normal astrocyte or GBM cell lines, and inhibition by rNDV-PTEN of cell viability and migration by inducing apoptotic cell death in U87-MG cells. PTEN (A) mRNA and (B) protein expression in normal astrocyte (CCF-STTG1) and GBM cell lines (U87-MG, T98G, Proneural X01 and Mesenchymal 83). CCF-STTG1 cells and U87-MG cells were infected rNDV or rNDV-PTEN 1 MOI for 36 h. (C) Cell viability assay performed using CCF-STTG1 cells and GBM cell lines with rNDV or rNDV-PTEN 1 MOI treatment using a Cell Counting Kit-8 Kit. (D) U87-MG cells were infected with rNDV or rNDV-PTEN and a Transwell assay was performed to assess cell migration. Cells migrated from the upper chamber to the lower chamber were stained with DAPI. Scale bar, 50 µm. (E) Apoptosis (DNA fragmentation) in U87-MG cells measured using TUNEL staining after virus infection. *P<0.05 vs. CCF-STTG1 or CON. PTEN, phosphatase and tensin homolog; GBM, glioblastoma; rNDV, recombinant Newcastle disease virus; MOI, multiplicity of infection; CON, control; n.s., not significant.

    Journal: Oncology Letters

    Article Title: Anticancer effect of the oncolytic Newcastle disease virus harboring the PTEN gene on glioblastoma

    doi: 10.3892/ol.2024.14752

    Figure Lengend Snippet: mRNA expression and protein expression of PTEN in normal astrocyte or GBM cell lines, and inhibition by rNDV-PTEN of cell viability and migration by inducing apoptotic cell death in U87-MG cells. PTEN (A) mRNA and (B) protein expression in normal astrocyte (CCF-STTG1) and GBM cell lines (U87-MG, T98G, Proneural X01 and Mesenchymal 83). CCF-STTG1 cells and U87-MG cells were infected rNDV or rNDV-PTEN 1 MOI for 36 h. (C) Cell viability assay performed using CCF-STTG1 cells and GBM cell lines with rNDV or rNDV-PTEN 1 MOI treatment using a Cell Counting Kit-8 Kit. (D) U87-MG cells were infected with rNDV or rNDV-PTEN and a Transwell assay was performed to assess cell migration. Cells migrated from the upper chamber to the lower chamber were stained with DAPI. Scale bar, 50 µm. (E) Apoptosis (DNA fragmentation) in U87-MG cells measured using TUNEL staining after virus infection. *P<0.05 vs. CCF-STTG1 or CON. PTEN, phosphatase and tensin homolog; GBM, glioblastoma; rNDV, recombinant Newcastle disease virus; MOI, multiplicity of infection; CON, control; n.s., not significant.

    Article Snippet: Proneural X01 and were cultured in DMEM/F12 (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10 ng/ml epidermal growth factor (cat. no. 236-EG; R&D Systems, Inc.), basic fibroblast growth factor (cat. no. 4114-TC; 5 ng/ml for Proneural X01 and 10 ng/ml for Mesenchymal 83; R&D Systems, Inc.).

    Techniques: Expressing, Inhibition, Migration, Infection, Viability Assay, Cell Counting, Transwell Assay, Staining, TUNEL Assay, Virus, Recombinant, Control